![]() Hyperpolarized experiments were carried out according to a standardized protocol, which showed a high repeatability (CV = 15%) and an average linewidth in the 1D- 13C NMR spectra of 2 ± 0.5 Hz. Binary classification and feature analysis was performed to make a separation model and to investigate differences between samples originating from normal and cancerous prostate tissue, respectively. Polar metabolite extracts were prepared, and hyperpolarized 1D- 13C NMR spectra were obtained from normal prostate (n = 19) and cancer tissue (n = 19) samples. Before euthanasia, mice were injected with glucose intraperitoneally (i.p.). Prostate and tumor tissue samples were acquired from transgenic adenocarcinomas of the mouse prostate (TRAMP) mice. Here we used hyperpolarized stable isotope resolved analysis (SIRA) to measure metabolite specific 13C isotopic enrichments in the central carbon metabolism of mouse prostate. Such hyperpolarized flux data may offer new approaches to tissue classification and biomarker identification that could be translated in vivo. Hyperpolarized 13C isotope resolved spectroscopy boosts NMR signal intensity, which improves signal detection and allows metabolic fluxes to be analyzed. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |